李周坤，男，河南信阳人，理学博士，副教授，硕士研究生导师，现为888.3net新浦京游戏888.3net新浦京游戏微生物学系教师。主要从事黏细菌资源开发与利用相关研究，基于黏细菌种质资源评价，聚焦黏细菌与土壤微生物互作及黏细菌糖酶生物学功能等基础研究，探索黏细菌在植物病害控制、复杂生物质与废弃物生物转化中的应用性；相关研究成果发表在ISME J、Nature Communications、Microbiome、Appl Environ Microbiol、Food Chem 等期刊上。担任Foods与Frontiers in Microbiology等期刊Guest Editor。
（1）教育经历：
2009/09-2015/12 ，888.3net新浦京游戏，微生物学，理学博士学位，导师: 崔中利 教授
2005/09-2009/07，河南科技学院，生物工程，学士学位
（2）工作经历：
2020/07-至今，888.3net新浦京游戏888.3net新浦京游戏微生物系，副教授；
2019/09-2020/06，888.3net新浦京游戏888.3net新浦京游戏微生物系，讲师；
2016/01-2019/06, 888.3net新浦京游戏，植物保护博士后流动站，师资博士后，合作导师: 张正光 教授
办公室：生科楼C7023；E-mail：zkl@njau.edu.cn
教学情况
本科教学：
（1）“粘细菌与农业”教授开放课程；（2）微生物生理学；
（3）普通微生物学；（4）基础微生物学实验，微生物生理学实验
指导本科生获奖：
（1）第五届全国大学生可再生能源优秀科技作品竞赛，三等奖，2023-3-2-1-205。
（2）第八届全国大学生生命科学竞赛（创新创业类），三等奖，GULSC2023CXCY1584。
（3）888.3net新浦京游戏第三届“振兴杯”创新创业大赛（高教赛道），三等奖
科研情况
黏细菌（Myxobacteria）在系统进化上属于phyla Myxococcota，是一类具有多细胞群体行为特征的微生物类群，具有复杂的生活史，能够形成类似于真菌的多形态子实体结构，被认为是高等的原核生物；黏细菌广泛分布于不同生境，能够以活的微生物细胞或者其他生物大分子作为食物获取营养，同时能够响应外界营养阈值进而调控营养细胞分化为抗逆性强的黏孢子，从而使黏细菌类群具有良好的环境适应性。黏细菌对于细菌和真菌的捕食特性及其生物质转化能力使其在土壤微生物食物网和土壤物质循环中扮演着重要的角色，表现出较强的进化适应性。本实验室以黏细菌为资源开展相关研究工作：
（1）黏细菌与植物病原菌互作机制Predator（myxobacteria）-Prey（plant pathogen）interactions
黏细菌作为土壤微生物食物网的顶端捕食者，其捕食特性和良好的土壤适应性使其在土壤微生物生态网络调控和植物病害控制方面具有重要的应用价值。本实验室通过鉴定捕食性黏细菌与植物病原菌互作过程中的关键因子，阐明捕食者（攻击）与猎物（防御）的互作机制及其适应性进化行为，解析黏细菌群体行为的生物学功能，为黏细菌应用于农业生产过程中植物病害控制提供基础。
（2）黏细菌来源的新酶资源开发与利用Industrial enzyme from myxobacteria and its application
黏细菌在生长过程中能够分泌不同类型的水解或氧化酶类，在黏细菌生长代谢和捕食过程具有特殊的生物学功能。本实验室主要针对黏细菌来源的病原菌细胞壁裂解酶CWDEs、淀粉酶、木聚糖酶等糖苷水解酶GHs和酯酶等，筛选具有优良催化特性的酶资源，解析酶催化的分子机制及其生物学功能，并评价其作为抗病因子、高附加值功能糖制备以及废弃物生物转化等方面的应用潜力。
相关科研项目：
1) 国家自然科学基金，面上项目，32270060，外膜型β-1,6-葡聚糖酶GluM在捕食性黏细菌发育中的作用机制，2023/01-2026/12，54万元，主持。
2) 国家自然科学基金，面上项目，32070027，外膜型β-1,6-葡聚糖酶在捕食性粘细菌识别真菌中的作用机制，2021/01-2024/12，58万元，主持。
3) 国家重点研发计划，绿色生物制造专项，2021YFC2103600，塑料高效生物解聚的关键技术，2021/12-2024/11，110万元，子课题主持。
4) 中央高校基本业务费，YDZX2023014，Myxococcus sp. MxC21与水稻互作调控水稻耐盐性的作用机制，2023/01-2023/12，7万元，主持。
5) 卫岗乳业有限公司横向课题，2022320122000695，乳制品全链条污染微生物、蛋白酶和脂肪酶的快速检测技术，2022.09-2023.12，100万元，参加。
6) 国家自然科学基金，青年科学基金项目，31700054，粘细菌新型麦芽糖淀粉酶AmyZ产高纯度麦芽糖的催化机制研究，2018/01-2020/12，24万元，主持。
7) 888.3net新浦京游戏-新疆农业大学联合基金项目，KYYJ202112，基于盐碱耐受性生防粘细菌的新疆棉花黄萎病绿色防控技术研发，2022/01-2022/12，5万元，主持。
8) 国家科技重大专项，转基因生物新品种培育：粘细菌来源的广谱抗水稻真菌病害基因的克隆和功能鉴定，2020ZX08009-04B，170万元，参加。
9) 中央高校基本业务费，KJQN201832，2018/01-2020/12，10万元，主持。
10) 中国博士后 科学基金面上项目，2016M591859，粘细菌Corallococus sp. EGB抗稻瘟病菌机制的研究，2016/01-2018/07，5万元，主持。
相关研究论文：
（1）黏细菌与植物病原菌互作与病害防控：
1) Lei Zhang, Chaonan Dong, Jihong Wang, Muxing Liu, Juying Wang, Jiexiong Hu, Lin Liu, Xinyu Liu, Chengyao Xia, Lingli Zhong, Yuqiang Zhao, Xianfeng Ye, Yan Huang, Jiaqin Fan, Hui Cao, Jingjing Wang, Yuezhong Li, Daniel Wall, Zhoukun Li*, Zhongli Cui*. Predation of oomycetes by myxobacteria via a specialized CAZyme system arising from adaptive evolution, ISME Journal, 2023.
2) Chengyao Xia, Yuqiang Zhao, Lei Zhang, Xu Li, Yang Cheng, Dongming Wang, Changsheng Xu, Mengyi Qi, Jihong Wang, Xiangrui Guo, Xianfeng Ye, Yan Huang, Danyu Shen, Daolong Dou, Hui Cao, Zhoukun Li*, Zhongli Cui*, Myxobacteria restrain Phytophthora invasion by scavenging thiamine in soybean rhizosphere via outer membrane vesicles-secreted thiaminase I, Nature Communications, 2023.
3) Xianfeng Ye#, Zhoukun Li#, Xue Luo, Wenhui Wang, Yongkai Li, Rui Li, Bo Zhang, Yan Qiao, Jie Zhou, Jiaqin Fan, Hui Wang, Yan Huang, Hui Cao, Zhongli Cui*, and Ruifu Zhang*, A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community. Microbiome, 2020, 8:49.
4) Zhoukun Li #, Xianfeng Ye #, Muxing Liu, Chengyao Xia, Lei Zhang, Xue Luo, Ting Wang, Yue Chen, Yuqiang Zhao, Yan Qiao, Yan Huang, Hui Cao, Xiangyang Gu, Jiaqin Fan, Zhongli Cui*, Zhengguang Zhang*, A novel outer membrane β-1,6-glucanase is deployed in the predation of fungi by myxobacteria. ISME Journal, 2019, 13(9), 2223-2235.
5) Lin Liu, Fengjuan Xu, Jinhui Lei, Peiwen Wang, Lei Zhang, Jihong Wang, Dongmei Mao, Xianfeng Ye, Huang Yan, Gang Hu*, Zhongli Cui, Zhoukun Li*, Genome analysis of a plasmid-bearing myxobacterim Myxococcus sp. strain MxC21 with salt-tolerant property. Frontiers in Microbiology. 2023, 14: 1250602.
6) Xue Luo, Yang Chen, Jihong Wang, Lin Liu, Yuqiang Zhao, Zhitong Jiang, Yanxin Wang, Zhoukun Li**, Lei Fu*, Zhongli Cui, Biocontrol potential of Burkholderia sp. BV6 against the rice blast fungus Magnaporthe oryzae. Journal of Applied Microbiology, 2022. 00:1-15.
7) Zhoukun Li #, Ting Wang #, Xue Luo, Xiangmin Li, Chengyao Xia, Yuqiang Zhao, Xianfeng Ye, Yan Huang, Xiangyang Gu, Hui Cao, Zhongli Cui*, Jiaqin Fan*. Biocontrol potential of Myxococcus sp. strain BS against bacterial soft rot of calla lily caused by Pectobacterium carotovorum. Biological Control, 2018, 126: 36-44.
8) Zhoukun Li, Xianfeng Ye, Peilin Chen, Kai Ji, Jie Zhou, Fei Wang, Weiliang Dong, Yan Huang, Zhengguang Zhang, Zhongli Cui*, Antifungal potential of Corallococcus sp. strain EGB against plant pathogenic fungi. Biological Control, 2017, 110: 10-17.
9) 李周坤，叶现丰，杨帆，黄彦，范加勤，王辉，崔中利*， 黏细菌捕食生物学研究进展及其在农业领域的应用潜力，888.3net新浦京游戏学报，2021，44(2): 208-216.
（2）黏细菌来源的新型工业酶资源挖掘与利用：
1) Enlong Shen, Xingchao Wang, Zhaoxi Lu, Fei Zhou, Weihua Ma, Zhongli Cui, Zhoukun Li*, Changyan Li*, Yongjun Lin*, Overexpression of a beta-1,6-glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut. Pest Management Science, 2023, 79: 2152-2162.
2) Zhoukun Li, Jihong Wang, Kanghui Lin, Muxing Liu, Juying Wang, Lei Zhang, Chengyao Xia, Lin Liu, Biying Zhang, Yunzhen Yangzong, Jian Han, Yuqiang Zhao, Xianfeng Ye, Yan Huang, Zhongli Cui*, Insights into the antifungal properties of myxobacteria outer membrane β-1,6-glucanase. Journal of Agricultural and Food Chemistry, 2023, 71: 9656–9666
3) Xu Li#, Lei Zhang#, Zhitong Jiang, Lin Liu, Jihong Wang, Lingli Zhong, Tao Yang, QinZhou, Weiliang Dong, Jie Zhou, Xianfeng Ye, Zhoukun Li*, Yan Huang, Zhongli Cui, A novel cold-active GH8 xylanase from cellulolytic myxobacterium and its application in food industry. Food Chemistry, 2022, 393:133463
4) Tao Yang, Lingli Zhong, Guangshuai Jiang, Lin Liu, Pei Wang, Yingxin Zhong, Qianru Yue, Lei Ouyang, Anqiang Zhang, Zhoukun Li*, Zhongli Cui, Dong Jiang, Qin Zhou*, Comparative study on bread quality and starch digestibility of normal and waxy wheat (Triticum aestivum L.) modified by maltohexaose producing α-amylases. Food Research International, 2022: 112034.
5) Yan Qiao, Xianfeng Ye, Lingli Zhong, Chengyao Xia, Lei Zhang, Fan Yang, Yongkai Li, Xiaodong Fang, Lei Fu, Yan Huang, Hui Cao, Zhoukun Li*, Zhongli Cui *, Yeast β-1,3-glucan production by an outer membrane β-1,6-glucanase: process optimization, structural characterization and immunomodulatory activity. Food & Function, 2022, 13: 3917-3930.
6) Lei Zhang, Lingli Zhong, Peiwen Wang, Lei Zhan, Yunzhen Yangzong, Tianqi He, Yi Liu, Dongmei Mao, Xianfeng Ye, Zhongli Cui, Yan Huang, Zhoukun Li*, Structural and functional properties of porous corn starch obtained by treating raw starch with AmyM. Foods, 2023, 12(17): 3157
7) Jihong Wang, Lei Zhang, Peiwen Wang，Jinhui Lei, Lingli Zhong, Lei Zhan, Xianfeng Ye, Yan Huang, Xue Luo*, Zhongli Cui, Zhoukun Li*, Identification and characterization of novel mal-to-oligosaccharide-forming amylase AmyCf from Cystobacter sp. strain CF23, Foods, 2023.
8) Yan Qiao,Chengyao Xia, Lin Liu, Lei Tang, Jihong Wang, Changsheng Xu, Juying Wang, Lei Zhang, Xianfeng Ye, Yan Huang, Dongmei Mao, Yongheng Liang, Zhoukun Li*, Zhongli Cui, Structural characterization and emulsifier property of yeast mannoprotein enzymatically prepared with a β-1,6-glucanase. LWT-Food Sci Technol, 2022, 168(1): 113898.
9) Lei Zhang#, Lingli Zhong#, Juying Wang, Yuqiang Zhao, Yiwei Zhang, Yitong Zheng, Weiliang Dong, Xianfeng Ye, Yan Huang, Zhoukun Li*, Zhongli Cui. Efficient hydrolysis of raw starch by a maltohexaose-forming α-amylase from Corallococcus sp. EGB. LWT-Food Sci Technol, 2021, 152:112361.
10) Chengyao Xia #, Lingli Zhong #, Juying Wang, Lei Zhang, Xiaopei Chen, Hangyan Ji, Shiyun Ma, Weiliang Dong, Xianfeng Ye, Yan Huang, Zhoukun Li*, Zhongli Cui. Structural and digestion properties of potato starch modified using an efficient starch branching enzyme AqGBE. Int J Biol Macromol, 2021. 184: 551-557.
11) Qiwen Fan, Lei Zhang, Chaonan Dong, Linli Zhong, Xiaodong Fang, Minghui Huan, Xianfeng Ye, Yan Huang, Zhoukun Li*, Zhongli Cui, Novel malto-oligosaccharide-producing amylase AmyAc from Archangium sp. strain AC19 and its catalytic properties. Starch-Starke, 2021.2100114:1-8.
12) Yanxin Wang, Muxing Liu, Xiaowen Wang, Linli Zhong, Guolong Shi, Xu Ye, Yangqing Li, Ruolin Li, Yan Huang, Xianfeng Ye, Zhoukun Li*, Zhongli Cui*. A novel β-1,3-glucanase Gns6 from rice possesses antifungal activity against Magnaporthe oryzae. J Plant Physiol. 2021, 265:153493
13) Xiaopei Chen, Lei Zhang, Xu Li, Yan Qiao, Yajuan Zhang, Yuqiang Zhao, Jian Chen, Xianfeng Ye, Yan Huang, Zhoukun Li*, Zhongli Cui, Impact of maltogenic α-amylase on the structure of potato starch and its retrogradation properties, Int J Biol Macromol, 2020,145:325-331.
14) Zhoukun Li, Chengyao Xia, Yanxin Wang, Xu Li, Yan Qiao, Chenyu Li, Jie Zhou, Lei Zhang, Xianfeng Ye, Yan Huang, Zhongli Cui*, Identification of an endo-chitinase from Corallococcus sp. EGB and evaluation its antifungal properties. Int J Biol Macromol, 2019, 132: 1235-1243.
15) Lei Zhang#, Zhoukun Li#, Yan Qiao, Yajuan Zhang, Wenwen Zheng, Yuqiang Zhao, Yan Huang, Zhongli Cui*, Improvement of the quality and shelf life of wheat bread by a maltohexaose producing α-amylase. J Cereal Sci, 2019, 87:165-171.
16) Zhoukun Li, Wenwen Zheng, Lei Zhang, Yanxin Wang, Yajuan Zhang, Yan Qiao, Xue Luo, Yan Huang, Zhongli Cui*, Gene expression and biochemical characterization of a GH77 4-α-glucanotransferase CcGtase from Corallococcus sp. EGB. Starch-Starke, 2019, 1800254.
17) Jie Zhou, Zhoukun Li, Han Zhang, Jiale Wu, Xianfeng Ye, Weiliang Dong, Min Jiang, Yan Huang*, Zhongli Cui*, A novel maltogenic amylase CoMA from Corallococcus sp. EGB catalyzes the conversion of maltooligosaccharides and soluble starch to maltose. Appl Environ Microbiol, 2018.
18) Zhoukun Li, Jiale Wu, Ting Wang, Wenwen Zheng, Yan Qiao, Yan Huang, Zhongli Cui*, Efficient production and characterization of maltohexaose-forming α-amylase AmyM secreted from methylotrophic yeast, Pichia pastoris. Starch-Starke, 2018, 70(11-12): 1700312.
19) Jie Zhou#, Zhoukun Li#, Jiale Wu, Lifeng Li, Ding Li, Xianfeng Ye, Xue Luo, Yan Huang, Zhongli Cui*, Hui Cao*, Functional analysis of a novel β-(1,3)-glucanase from Corallococcus sp. EGB containing a fascin-like module. Appl Environ Microbiol, 2017, 83(16): e01016-17.
20) Zhoukun Li#, Kai Ji#, Jie zhou, Xianfeng Ye, Ting Wang, Xue Luo, Yan Huang, Hui Cao, Zhongli Cui*, Yi Kong*, A debranching enzyme IsoM of Corallococcus sp. strain EGB with potential in starch processing. Int J Biol Macromol, 2017, 105(1): 1300-1309.
21) Zhoukun Li, JialeWu, Biying Zhang, Fei Wang, Xianfeng Ye, Yan Huang, Qiang Huang, Zhongli Cui*, AmyM, a Novel Maltohexaose-Forming α-Amylase from Corallococcus sp. Strain EGB. Appl Environ Microbiol, 2015, 8(6): 1977-1987.
相关发明专利：
1) 崔中利，李周坤，黄彦 一种珊瑚球菌及其应用. 申请号：ZL 201310028459.8 (已授权)。
2) 崔中利，李周坤，黄彦 一种α-淀粉酶及其基因、含有该基因的工程菌及其应用，申请号：ZL 201310043628.5 (已授权)。
3) 崔中利，李周坤，张碧莹，叶现丰，黄彦 一种新的β-1,6-葡聚糖酶及其基因和应用. 申请号：ZL 201510816987.9 (已授权)。
4) 崔中利，李周坤，冀凯，黄彦 一种异淀粉酶及其基因、含有该基因的工程菌及其应用，申请号：ZL 201610368731.0 (已授权)。
5) 崔中利，周杰，李周坤，吴家乐，黄彦 一种麦芽糖淀粉酶及其基因、含有该基因的工程菌及其应用，申请号：ZL201610158814.7 (已授权)。
6) 崔中利，李周坤，王婷，罗雪，黄彦 一株捕食植物病原细菌的叶柄粘球菌及其在细菌性病害生物防治中的应用，申请号：ZL201711363218.3 (已授权)。
7) 崔中利，李周坤，陈晓培，夏丞垚，黄彦 一种淀粉分支酶及其基因、含有该基因的工程菌及其应用，申请号：ZL201911127851.1 (已授权)。
8) 崔中利，乔燕，叶现丰，李周坤，曹慧一种利用β-1,6葡聚糖酶联产酵母葡聚糖、甘露糖蛋白和酵母提取物的方法，申请号：ZL201910220003.9 (已授权)。
9) 崔中利，李周坤，李旭，钟玲丽，一种耐冷木聚糖酶及其基因、含有该基因的工程菌及其应用，申请号：ZL202210125774.1 (已授权)。
10) 崔中利，李周坤，张蕾，叶现丰，一株捕食性原囊菌及其在植物病害生物防治中的应用，申请号：ZL202210755118.X (已授权)。
11) 崔中利，李周坤，钟玲丽，张蕾，两种淀粉水解能力增强的麦芽六糖淀粉酶突变体及其制备方法和应用，申请号：202210408329.6。
12) 李周坤，张蕾，崔中利，王吉宏，叶现丰，黄彦，一种黏细菌来源的β-1,3-葡聚糖酶及其组合物在植物疫霉病生物防治中的应用，申请号：202310149719.0。
13) 李周坤，柳林，张蕾，崔中利，王菊颖，倪兰，一株耐盐性黏球菌及其应用，申请号：2023104260492。
